Apolipoprotein L1-specific antibodies detect endogenous APOL1 inside the endoplasmic reticulum and on the plasma membrane of podocytes
SJ Scales, N Gupta, AM De Mazière… - Journal of the …, 2020 - journals.lww.com
Background APOL1 is found in human kidney podocytes and endothelia. Variants G1 and
G2 of the APOL1 gene account for the high frequency of nondiabetic CKD among African
Americans. Proposed mechanisms of kidney podocyte cytotoxicity resulting from APOL1
variant overexpression implicate different subcellular compartments. It is unclear where
endogenous podocyte APOL1 resides, because previous immunolocalization studies
utilized overexpressed protein or commercially available antibodies that crossreact with …
G2 of the APOL1 gene account for the high frequency of nondiabetic CKD among African
Americans. Proposed mechanisms of kidney podocyte cytotoxicity resulting from APOL1
variant overexpression implicate different subcellular compartments. It is unclear where
endogenous podocyte APOL1 resides, because previous immunolocalization studies
utilized overexpressed protein or commercially available antibodies that crossreact with …