[HTML][HTML] Independent regulation of Piezo1 activity by principal and intercalated cells of the collecting duct
K Pyrshev, A Atamanchuk-Stavniichuk… - Journal of Biological …, 2024 - ASBMB
The renal collecting duct is continuously exposed to a wide spectrum of fluid flow rates and
osmotic gradients. Expression of a mechanoactivated Piezo1 channel is the most prominent
in the collecting duct. However, the status and regulation of Piezo1 in functionally distinct
principal and intercalated cells (PCs and ICs) of the collecting duct remain to be determined.
We used pharmacological Piezo1 activation to quantify Piezo1-mediated [Ca 2+] i influx and
single-channel activity separately in PCs and ICs of freshly isolated collecting ducts with …
osmotic gradients. Expression of a mechanoactivated Piezo1 channel is the most prominent
in the collecting duct. However, the status and regulation of Piezo1 in functionally distinct
principal and intercalated cells (PCs and ICs) of the collecting duct remain to be determined.
We used pharmacological Piezo1 activation to quantify Piezo1-mediated [Ca 2+] i influx and
single-channel activity separately in PCs and ICs of freshly isolated collecting ducts with …