[HTML][HTML] Phosphorylation decreases ubiquitylation of the thiazide-sensitive cotransporter NCC and subsequent clathrin-mediated endocytosis
LL Rosenbaek, MLA Kortenoeven, TS Aroankins… - Journal of Biological …, 2014 - ASBMB
The thiazide-sensitive sodium chloride cotransporter, NCC, is the major NaCl transport
protein in the distal convoluted tubule (DCT). The transport activity of NCC can be regulated
by phosphorylation, but knowledge of modulation of NCC trafficking by phosphorylation is
limited. In this study, we generated novel tetracycline-inducible Madin-Darby canine kidney
type I (MDCKI) cell lines expressing NCC to examine the role of NCC phosphorylation and
ubiquitylation on NCC endocytosis. In MDCKI-NCC cells, NCC was highly glycosylated at …
protein in the distal convoluted tubule (DCT). The transport activity of NCC can be regulated
by phosphorylation, but knowledge of modulation of NCC trafficking by phosphorylation is
limited. In this study, we generated novel tetracycline-inducible Madin-Darby canine kidney
type I (MDCKI) cell lines expressing NCC to examine the role of NCC phosphorylation and
ubiquitylation on NCC endocytosis. In MDCKI-NCC cells, NCC was highly glycosylated at …