[HTML][HTML] C-terminally truncated, kidney-specific variants of the WNK4 kinase lack several sites that regulate its activity
AR Murillo-de-Ozores, A Rodríguez-Gama… - Journal of Biological …, 2018 - ASBMB
WNK lysine-deficient protein kinase 4 (WNK4) is an important regulator of renal salt
handling. Mutations in its gene cause pseudohypoaldosteronism type II, mainly arising from
overactivation of the renal Na+/Cl− cotransporter (NCC). In addition to full-length WNK4, we
have observed faster migrating bands (between 95 and 130 kDa) in Western blots of kidney
lysates. Therefore, we hypothesized that these could correspond to uncharacterized WNK4
variants. Here, using several WNK4 antibodies and WNK4−/− mice as controls, we showed …
handling. Mutations in its gene cause pseudohypoaldosteronism type II, mainly arising from
overactivation of the renal Na+/Cl− cotransporter (NCC). In addition to full-length WNK4, we
have observed faster migrating bands (between 95 and 130 kDa) in Western blots of kidney
lysates. Therefore, we hypothesized that these could correspond to uncharacterized WNK4
variants. Here, using several WNK4 antibodies and WNK4−/− mice as controls, we showed …