Protein phosphatase 1 inhibitor–1 mediates the cAMP-dependent stimulation of the renal NaCl cotransporter
D Penton, S Moser, A Wengi, J Czogalla… - Journal of the …, 2019 - journals.lww.com
Background A number of cAMP-elevating hormones stimulate phosphorylation (and hence
activity) of the NaCl cotransporter (NCC) in the distal convoluted tubule (DCT). Evidence
suggests that protein phosphatase 1 (PP1) and other protein phosphatases modulate NCC
phosphorylation, but little is known about PP1's role and the mechanism regulating its
function in the DCT. Methods We used ex vivo mouse kidney preparations to test whether a
DCT-enriched inhibitor of PP1, protein phosphatase 1 inhibitor–1 (I1), mediates cAMP's …
activity) of the NaCl cotransporter (NCC) in the distal convoluted tubule (DCT). Evidence
suggests that protein phosphatase 1 (PP1) and other protein phosphatases modulate NCC
phosphorylation, but little is known about PP1's role and the mechanism regulating its
function in the DCT. Methods We used ex vivo mouse kidney preparations to test whether a
DCT-enriched inhibitor of PP1, protein phosphatase 1 inhibitor–1 (I1), mediates cAMP's …