Specific serum IgA, IgG and IgM antibody determination by a modified indirect ELISA-technique in primary and recurrent herpes simplex virus infection

P Juto, B Settergren - Journal of virological methods, 1988 - Elsevier
Twenty-three patients with a herpetic infection as diagnosed by a positive culture of herpes
simplex virus (HSV) were studied with respect to serological responses of IgA, IgG and IgM
antibodies in paired serum samples by an indirect (sandwich) enzyme linked
immunosorbent assay (ELISA). Eight of the patients had a primary infection and 15 a
recurrent one. In the ELISA test a detergent treated cell lysate of HSV type 1 was used as
antigen. In the IgM assay all sera were pretreated with antihuman IgG with the purpose to …