Isobaric stable isotope labeling using, for example, tandem mass tags (TMTs) is increasingly
being applied for large-scale proteomic studies. Experiments focusing on proteoform
analysis in drug time course or perturbation studies or in large patient cohorts greatly benefit
from the reproducible quantification of single peptides across samples. However, such
studies often require labeling of hundreds of micrograms of peptides such that the cost for
labeling reagents represents a major contribution to the overall cost of an experiment. Here …