Transcriptomic profiling of human choriodecidua during term labor: inflammation as a key driver of labor
GL Stephen, S Lui, SA Hamilton… - American journal of …, 2015 - Wiley Online Library
GL Stephen, S Lui, SA Hamilton, CL Tower, LK Harris, A Stevens, RL Jones
American journal of reproductive immunology, 2015•Wiley Online LibraryProblem Inflammation is a driver of labor in myometrium and cervix; however, the
involvement of decidua is poorly defined. We have reported decidual leukocyte infiltration
prior to and during labor; the regulators of these inflammatory processes are unknown.
Method of study Choriodecidua RNA obtained after term labor or elective cesarean delivery
was applied to Affymetrix GeneChips. Pathway analysis and gene validation were
performed. Results Extensive inflammatory activation was identified in choriodecidua …
involvement of decidua is poorly defined. We have reported decidual leukocyte infiltration
prior to and during labor; the regulators of these inflammatory processes are unknown.
Method of study Choriodecidua RNA obtained after term labor or elective cesarean delivery
was applied to Affymetrix GeneChips. Pathway analysis and gene validation were
performed. Results Extensive inflammatory activation was identified in choriodecidua …
Problem
Inflammation is a driver of labor in myometrium and cervix; however, the involvement of decidua is poorly defined. We have reported decidual leukocyte infiltration prior to and during labor; the regulators of these inflammatory processes are unknown.
Method of study
Choriodecidua RNA obtained after term labor or elective cesarean delivery was applied to Affymetrix GeneChips. Pathway analysis and gene validation were performed.
Results
Extensive inflammatory activation was identified in choriodecidua following labor, predominantly upregulation of genes regulating leukocyte trafficking and cytokine signalling. Genes governing cell fate, tissue remodelling, and translation were also altered. Upregulation of candidate genes (ICAM1, CXCR4, CD44, TLR4, SOCS3, BCL2A, and IDO) was confirmed. NFκB, STAT1&3, HMGB1, and miRNA‐21, miRNA‐46, miRNA‐141, and miRNA‐200 were predicted upstream regulators.
Conclusion
This study confirms inflammatory processes are major players in labor events in choriodecidua, as in other gestational tissues. Suppressing uterine inflammation is likely to be critical for arresting premature labor.
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