[HTML][HTML] Acetylation at lysine 183 of progesterone receptor by p300 accelerates DNA binding kinetics and transactivation of direct target genes
HH Chung, SK Sze, ASL Tay, VCL Lin - Journal of Biological Chemistry, 2014 - ASBMB
The identification of lysine acetylation of steroid hormone receptors has previously been
based on the presence of consensus motif (K/R) XKK. This study reports the discovery by
mass spectrometry of a novel progesterone receptor acetylation site at Lys-183 that is not in
the consensus motif. In vivo acetylation and mutagenesis experiments revealed that Lys-183
is a primary site of progesterone receptor (PR) acetylation. Lys-183 acetylation is enhanced
by p300 overexpression and abrogated by p300 gene silencing, suggesting that p300 is the …
based on the presence of consensus motif (K/R) XKK. This study reports the discovery by
mass spectrometry of a novel progesterone receptor acetylation site at Lys-183 that is not in
the consensus motif. In vivo acetylation and mutagenesis experiments revealed that Lys-183
is a primary site of progesterone receptor (PR) acetylation. Lys-183 acetylation is enhanced
by p300 overexpression and abrogated by p300 gene silencing, suggesting that p300 is the …