[HTML][HTML] Role of unc5b in retinal neovascularization in mice with oxygen-induced retinopathy
D Liu, XB Xia, XL Xu, XF Tian… - International Journal of …, 2011 - ncbi.nlm.nih.gov
D Liu, XB Xia, XL Xu, XF Tian, L Shang
International Journal of Ophthalmology, 2011•ncbi.nlm.nih.govAIM To explore the role of unc5b in retinal neovascularization in murine oxygen-induced
retinopathy (OIR). METHODS On postnatal 7 (P7), C57BL/6J mice were exposed to
75%±2% oxygen for 5 days. On postnatal 12 (P12), the mice were brought back to the room
air (21% oxygen) to induce retinal neovascularization. Western blot analysis was performed
to examine the temporal expression of unc5b in murine retinas. Double staining for unc5b
and isolectin B4 were employed to determine the location of unc5b in murine retinas. The …
retinopathy (OIR). METHODS On postnatal 7 (P7), C57BL/6J mice were exposed to
75%±2% oxygen for 5 days. On postnatal 12 (P12), the mice were brought back to the room
air (21% oxygen) to induce retinal neovascularization. Western blot analysis was performed
to examine the temporal expression of unc5b in murine retinas. Double staining for unc5b
and isolectin B4 were employed to determine the location of unc5b in murine retinas. The …
Abstract
AIM
To explore the role of unc5b in retinal neovascularization in murine oxygen-induced retinopathy (OIR).
METHODS
On postnatal 7 (P7), C57BL/6J mice were exposed to 75%±2% oxygen for 5 days. On postnatal 12 (P12), the mice were brought back to the room air (21% oxygen) to induce retinal neovascularization. Western blot analysis was performed to examine the temporal expression of unc5b in murine retinas. Double staining for unc5b and isolectin B4 were employed to determine the location of unc5b in murine retinas. The effect of unc5b on retinal neovascularization was evaluated by intravitreal injection of unc5b-FC in mice with OIR. Retinal neovascularization was measured by counting neovascular cell nuclei above the internal limiting membrane and by angiography of flat-mounted retinas perfused with fluorescein dextran.
RESULTS
Compared to age-matched normal mice, the expression of unc5b was significantly increased in retinas of OIR mice on P17 and P21. Unc5b was apparently expressed in retinal vessels of OIR while being negative in normal retinal vessels. Retinal neovascularization in eyes injected with unc5b-FC was significantly reduced.
CONCLUSION
Unc5b-FC can effectively inhibit retinal neovascularization induced by OIR. It may serve as a powerful and novel therapy for ischemia-induced retinal disease.
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