[HTML][HTML] Neuropilin-1 binds vascular endothelial growth factor 165, placenta growth factor-2, and heparin via its b1b2 domain
R Mamluk, Z Gechtman, ME Kutcher… - Journal of Biological …, 2002 - ASBMB
Neuroplin-1 (NRP1), a receptor for vascular endothelial growth factor (VEGF) family
members, has three distinct extracellular domains, a1a2, b1b2, and c. To determine the
VEGF 165 and placenta growth factor 2 (PlGF-2)-binding sites of NRP1, recombinant NRP1
domains were expressed in mammalian cells as Myc-tagged, soluble proteins, and used in
co-precipitation experiments with 125 I-VEGF 165 and 125 I-PlGF-2. Anti-Myc antibodies
immunoprecipitated 125 I-VEGF 165 and 125 I-PlGF-2 in the presence of the b1b2 but not of …
members, has three distinct extracellular domains, a1a2, b1b2, and c. To determine the
VEGF 165 and placenta growth factor 2 (PlGF-2)-binding sites of NRP1, recombinant NRP1
domains were expressed in mammalian cells as Myc-tagged, soluble proteins, and used in
co-precipitation experiments with 125 I-VEGF 165 and 125 I-PlGF-2. Anti-Myc antibodies
immunoprecipitated 125 I-VEGF 165 and 125 I-PlGF-2 in the presence of the b1b2 but not of …
