Here, we describe the construction of a set of binary adenovirus vectors encoding for a
tetracycline-regulatable expression cassette and the Tet-ON or the Tet-OFF transcriptional
activator proteins from a single viral chromosome. The rabies virus glycoprotein was cloned
into the E1 region and the tetracycline activator proteins were inserted in both orientation in
place of the E3 region. To further restrict background transcription, we also introduced a Lac
repressor protein based roadblock to transcription elongation. To make the system more …