[HTML][HTML] Sustained calcium entry through P2X nucleotide receptor channels in human airway epithelial cells
A Zsembery, AT Boyce, L Liang, J Peti-Peterdi… - Journal of Biological …, 2003 - ASBMB
Purinergic receptor stimulation has potential therapeutic effects for cystic fibrosis (CF). Thus,
we explored roles for P2Y and P2X receptors in stably increasing [Ca 2+] i in human CF (IB3-
1) and non-CF (16HBE14o−) airway epithelial cells. Cytosolic Ca 2+ was measured by
fluorospectrometry using the fluorescent dye Fura-2/AM. Expression of P2X receptor (P2XR)
subtypes was assessed by immunoblotting and biotinylation. In IB3-1 cells, ATP and other
P2Y agonists caused only a transient increase in [Ca 2+] i derived from intracellular stores in …
we explored roles for P2Y and P2X receptors in stably increasing [Ca 2+] i in human CF (IB3-
1) and non-CF (16HBE14o−) airway epithelial cells. Cytosolic Ca 2+ was measured by
fluorospectrometry using the fluorescent dye Fura-2/AM. Expression of P2X receptor (P2XR)
subtypes was assessed by immunoblotting and biotinylation. In IB3-1 cells, ATP and other
P2Y agonists caused only a transient increase in [Ca 2+] i derived from intracellular stores in …